Diatom Test Slide version 2.0 - Test Slides

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Diatom Test Slide version 2.0

Instruction Manual: Venture into the Micro and Nano structures!
Copyright © 2018-2019 testslides.com, diatomshop.com , diatomlab.com

PREMISE:

Visit the Home Page to read The 10 REASONS DIATOM LAB PREPARATIONS ARE SO INNOVATIVE! (You can save PDF file "The 10 reasons Diatom Lab preparations are so innovative" by this link)


This high-end test microscope slide contains 5 cleaned, selected and micromanipulated Diatom species with striae, areolae and poroids that can be RESOLVED or DETECTED through a light microscope.

It is extremely
useful to
A)
compare objectives, condensers, microscopes and optical microscope illumination techniques such as bright field, oblique illumination, differential interference contrast (DIC), phase contrast, dark field, polarized light, Rheinberg illumination and Hoffman modulation contrast!
B)
practice using your microscopes at their highest levels!
C)
determine the resolving power of your microscope (the resolving power of a microscope is measured by its ability to differentiate two lines or points in an object)!
D) examine the
variations in contrast and resolution by regulating the condenser aperture diaphragm
E) understand the importance of correction collar for minimazing spherical aberration
F) examine the variations in resolution by using different wavelengths of light

This is a
STANDARDIZED HIGH-END TEST, in fact each microscope slide has the same production characteristics!
Mounted in Diatom Cubed, high refractive index diatom mountant (
refractive index > 1,7)!

This product has been
thoroughly tested before going to market, using dry, oil immersion and double immersion light microscopy (also the last two Diatom species have been tested on Zeiss Axio Imager.A2 research microscope using the Zeiss Achromatic-aplanatic condenser 1,4 H D Ph DIC, the Zeiss Objective 63x/1,4 Oil DIC ∞/0,17 M27 and other high aperture (N.A. 1,3 and 1.4) Zeiss M27 objectives)

TECHNICAL SUGGESTIONS:

Diatom number 1
Species: Stauroneis phoenicenteron (Nitzsch) Ehrenberg
Striae in 10 µm: 12-15 longitudinal
Details to resolve: areolae, forming the striae
Suggested techniques:
Dry microscope objectives in Bright field, or Bright field with Oblique illumination, or Dark field illumination, or Phase contrast, or Differential interference contrast (DIC)

Diatom number 2
Species: Gyrosigma attenuatum (Kützing) Rabenhorst
Striae in 10 µm: 13-17 longitudinal
Details to resolve:  areolae, forming the striae
Suggested techniques:
Dry or Oil immersion microscope objectives in Bright field, or Bright field with Oblique illumination, or Dark field illumination, or Phase contrast, or Differential interference contrast (DIC)

Diatom number 3
Species: Gyrosigma reimeri F.A.S.Sterrenburg
Striae in 10 µm: 18-22 longitudinal
Details to resolve:  areolae, forming the striae
Suggested techniques:
Oil immersion objectives  in Bright field, or Bright field with Oblique illumination, or Dark field illumination, or Phase contrast, or Differential interference contrast (DIC)

Diatom number 4  
Species: Navicula oblonga (Kützing) Kützing
Details to detect: areolae (lineolae), forming the striae
Areolae (lineolae) in 10 µm: 48-50, see Scanning Electron Microscope (SEM) measurements in the photo gallery
AVERAGE DISTANCE BETWEEN areolae (lineolae): 0,14 µm, see Scanning Electron Microscope (SEM) measurements in the photo gallery
The theoretical limit of resolution of most light  microscopes  is ~ 0.2 μm, but these areolae (lineolae) can be detected by the techniques below, thanks to Diatom Cubed high refractive index mountant!

Recommended microscope objectives: oil-immersion 63 or 100x objectives having a  good or excellent numerical aperture (starting from 1,2; better: 1,3 or 1,4)
Suggested techniques:
Double immersion  = Oil immersion objective and Oil immersion condenser and:
Polarized light (the polarizers should be oriented perpendicular to each other = maximum level of extinction);
or Circular oblique illumination (C.O.L.) with polarized light;
orDark field illumination using an immersion dark field condenser (better 1,2/1,4);
or Differential interference contrast (DIC);
or UV illumination: in this case highly  specialized laboratory  facilities are required (it is dangerous for the eyes, it requires the use of special protection devices, accessories and cameras. Please refer to the operating manual  of your instruments);
or some variants of  the  Differential interference contrast (such as AVEC-DIC, the Allen Video-enhanced Contrast);
or IRC (Interference reflection contrast technique)

Diatom number 5
Species: Pinnularia nobilis (Ehrenberg) Ehrenberg
Details to detect:
Poroids
AVERAGE DISTANCE BETWEEN POROIDS: 0,11 µm (NEW MORE DIFFICULT SAMPLE!), see Scanning Electron Microscope (SEM) measurements in the photo gallery
Again, the theoretical limit of resolution of most light  microscopes  is ~ 0.2 μm, but these poroids can be detected by the techniques below, thanks to Diatom Cubed high refractive index mountant!

Recommended microscope objectives: oil-immersion 63 or 100x objectives having a very good or excellent numerical aperture (1,3 or 1,4)
Suggested techniques:
Double immersion = Oil immersion objective and Oil immersion condenser and:
Polarized light (the polarizers should be oriented perpendicular to each other = maximum level of extinction) with possibly oblique illumination;
or Immersion dark field condenser (better 1,2/1,4) with Polarized light (the polarizers should be oriented perpendicular to each other = maximum level of extinction)


 
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